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Selective Adsorption of an Antibody onto a Plasma-Polymerized Film for Protein Patterning

Atsunori HIRATSUKA, Naoya MURATA, Hitoshi MUGURUMA, Kazunari MATSUMURA

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Summary :

Techniques for patterned modification of substrate surfaces are important for forming microarrays on protein chips. A hexamethyldisiloxane plasma-polymerized film (HMDS PPF) was deposited on a glass substrate and the resulting surface was partially modified by subsequent nitrogen plasma treatment with a patterned shadow mask. When surface adsorption of an antibody protein (F(ab')2 fragment of anti-human immunoglobulin G) was visualized by fluorescence microscopy, distinct 8080 µm2 square spots were observed, surrounded by a non-fluorescent 80 µm-wide grid. This pattern could be attributed to proteins selectively adsorbed onto the nitrogen plasma-treated regions but not onto the surface of pristine HMDS PPF. This provided a simple fabrication method of protein patterning.

Publication
IEICE TRANSACTIONS on Electronics Vol.E91-C No.6 pp.978-980
Publication Date
2008/06/01
Publicized
Online ISSN
1745-1353
DOI
10.1093/ietele/e91-c.6.978
Type of Manuscript
LETTER
Category
Organic Molecular Electronics

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