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Plasma Polymerization for Protein Patterning: Reversible Formation with Fullerene Modification

Hayato TAKAHASHI, Naoya MURATA, Hitoshi MUGURUMA

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Summary :

Partial plasma polymerization for coexistence of hydrophobic/hydrophilic area in several ten micrometer size is the typical technique for protein patterning. A hydrophobic hexamethyldisiloxane plasma-polymerized film (HMDS PPF) was deposited on a glass substrate and this surface was partially modified by subsequent nitrogen plasma treatment (hydrophilic surface, HMDS-N PPF) with a patterned shadow mask. An antibody protein (F(ab')2 fragment of anti-human immunoglobulin G) was selectively adsorbed onto the HMDS-N area and was not adsorbed onto the HMDS area. Distinct 8080 µm2 square spots surrounded by a non-protein adsorbed 80 µm-wide grid were observed. Then, when the protein modified by fullerene was used, the reversible patterning was obtained. This indicated that the modification by fullerene changed the hydrophilic nature of F(ab')2 protein to hydrophobic one, as a result, the modified protein was selectively adsorbed onto hydrophobic area.

Publication
IEICE TRANSACTIONS on Electronics Vol.E93-C No.2 pp.211-213
Publication Date
2010/02/01
Publicized
Online ISSN
1745-1353
DOI
10.1587/transele.E93.C.211
Type of Manuscript
LETTER
Category
Organic Molecular Electronics

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