Stimulus response caused by low-frequency electromagnetic exposure can be used to realize effective medical treatments. However, it is well known that the membrane potential of nerve cells exhibits nonlinearity, particularly in the case of modulated signals, it is important to analyze the nonlinear stimulation effect based on a nerve cell model. This paper adopts the Frankenhaeuser-Huxley (FH) model as the nerve cell model, and evaluates the stimulus response based on the FH model when nerve cells are exposed to low-frequency modulated radio signals such as On-Off Keying (OOK) modulation. This paper investigated the nonlinear effect of the stimulus intensity from the viewpoint of the spike frequency with different modulation parameter values including the induced amplitude and duty cycle. It was confirmed that action potential (AP) rate was higher with OOK-type radio signals than with unmodulated signals.