The purpose of our study is to estimate the imaging of ischemic myocardial muscles in rats. The magnetocardiograms (MCG) of rats were measured by a 12-channel high resolution gradiometer, which consisted of 5 mm diameter pick-up coils with a 7.5 mm distance between each coil. MCGs of seven male rats were measured in a magnetically shielded room pre and post coronary artery occlusion. The source imaging was estimated by minimum norm estimation (MNE). Changes of the current source imaging pre- and post coronary artery occlusion were clarified. As a result, in the ST segment, the current distribution significantly increased at the ischemic area. In the T wave, the direction of the current distribution clearly shifted to the left thorax. We proved that the increased area of the current distribution in the ST segment was related to the ischemic area of the ventricular muscles.