A radio acoustic sounding system (RASS) is used to remotely measure the atmospheric temperature profiles, since the speed of the acoustic pulse can be measured from the ground by means of a Doppler radar. A theoretical analysis on received signal characteristics of a RASS which uses acoustic pulse-modulated and radio continuous waves has been made by deriving the scattered field from weak refractivity variations produced by an acoustic pulse. The analysis has revealed the influence of horizontal winds for temperature measurement by a RASS as follows:[1] The shift frequencies of return signals of a RASS coincide with Doppler frequencies only under low wind condition.[2] The shift frequencies of return signals of a RASS coincide with acoustic frequencies transmitted under strong wind condition.Measured results by a JRC RASS confirmed the results of the theoretical analysis.