Photoacoustic (PA) spectra on the 3, 4, 9, 10-perylenetetracarboxylic dianhydride (PTCDA) films deposited by the vacuum evaporation were measured. The films have layered structures constructed from the perylene molecule plane structures. The crystal quality depended on the deposited substrate and the photoacoustic spectroscopy (PAS) seems to be the very useful tools to evaluate these properties from the non-radiative features. The films deposited on the three different substrate had the almost same PL spectra, but the films deposited on the glass substrate had the large non-radiative peaks in the PA spectra contrary to the films deposited on the alumina or crystal Si (100) those had the non-radiative peaks only observed at the short wavelength region.