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[Author] Pratima R. SOLANKI(1hit)

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  • Conducting Polymer Based Nucleic Acid Sensor for Environment Monitoring

    Bansi Dhar MALHOTRA  Nirmal PRABHAKAR  Pratima R. SOLANKI  

     
    INVITED PAPER

      Vol:
    E91-C No:12
      Page(s):
    1889-1893

    Nucleic acid sensor based on polyaniline has been fabricated by covalently immobilizing double stranded calf thymus (dsCT) DNA onto perchlorate (ClO- 4) doped polyaniline (PANI) film deposited onto indium-tin-oxide (ITO) glass plate using 1-(3-(dimethylamino) propyl)-3-ethylcarbodiimide hydrochloride (EDC)/N-hydroxyl succinimide (NHS) chemistry. These dsCT-DNA-PANI/ITO and PANI/ITO electrodes have been characterized using square wave voltammetry, electrochemical impedance, and Fourier-transform-infra-red (FTIR) measurements. This disposable dsCT-DNA-PANI/ITO bioelectrode is stable for about four months, can be used to detect arsenic trioxide (0.1 ppm) in 30 s.