Quantum Cellular Automaton (QCA), which is one of the candidates for future integrable electron devices, is investigated from the viewpoint of operation temperature. The extended Hubbard model which can extract the physical essence of QCA is used to analyze the inter-cell interaction for a layered cell structure. We found that the operation energy estimated from the energy gap between the ground state and excited states of the layered structure is large enough to allow room temperature operation even if the size of quantum dot is as large as 500. The layered cell structure is found to be suitable for a memory application.