This paper develops the first prototype hardware for a TDD two-way multi-hop relay network with MIMO network coding. Since conventional wireless multi-hop relay networks have the drawback of low data rate, TDD two-way multi-hop relay networks have been studied as a solution to realize high data rate recently. In these networks, forward and backward streams are spatially multiplexed by using interference cancellation techniques such as MIMO beamforming or MIMO network coding. In this paper, a demonstration system for the TDD two-way multi-hop relay network with MIMO network coding (called 2-way relay network hereafter) is developed using the prototype hardware. In the demonstration system, each transmitter and receiver performs network coded broadcast and MIMO multiple access, respectively. By using the demonstration system, network throughput is measured in an indoor environment to prove the realization and effectiveness of the 2-way relay network. From the results of network throughput, it is found that the 2-way relay network can achieve high network throughput approaching theoretical upper bound even in low average end-to-end SNR area where network throughput of the direct link degrades severely. From these results, the realization and effectiveness of the 2-way relay network can be proved in the real indoor environment.