One-dimensional photonic crystal (PC) with alternating layers of TiO2 and SiO2 was fabricated with spin coating and low temperature baking, resulting in a successful tuning of the PC stop band so as to block the amplified spontaneous emission (ASE) of a π-conjugate polymer film. Single PC as a substrate, not a cavity with two PC's, of the polymer film was sufficient to shift the tangential ASE to the energy at PC stop band edge, indicating that the tangential ASE propagating along the interface was modulated by its evanescent-field tail in the PC, which opens the new pathway for low-threshold coherent luminescence from an ultrathin π-conjugate polymer film with ultimate mode volume.