In this letter, we propose a novel frequency-domain equalization (FDE) scheme for single-carrier multiple-input multiple-output (MIMO) systems over time-varying channels. Based on frequency-domain decision-feedback equalization (FD-DFE), we design a feedforward filter with constraint such that the equalization can be easily realized segment-by-segment with the help of the overlap-save (OLS) method. Since the segment length and block length can be designed independently, our proposal sets relatively short segment length to obtain good performance in time-varying environments, and very long block length to achieve high spectral efficiency. Furthermore, we present two scenarios in the design of filters for MIMO systems.