In recent years, measuring biomagnetic fields in the Earth’s field by differential measurements of scalar-mode OPMs have been actively attempted. In this study, the sensitivity of the scalar-mode OPMs under the geomagnetic environment in the laboratory was studied by numerical simulation. Although the noise level of the scalar-mode OPM in the laboratory environment was calculated to be 104 pT/$\sqrt{\mathrm{Hz}}$, the noise levels using the first-order and the second-order differential configurations were found to be 529 fT/cm/$\sqrt{\mathrm{Hz}}$ and 17.2 fT/cm2/$\sqrt{\mathrm{Hz}}$, respectively. This result indicated that scalar-mode OPMs can measure very weak magnetic fields such as MEG without high-performance magnetic shield roomns. We also studied the operating conditions by varying repetition frequency and temperature. We found that scalar-mode OPMs have an upper limit of repetition frequency and temperature, and that the repetition frequency should be set below 4 kHz and the temperature should be set below 120°C.