Round-trip time (RTT) is an important performance metric. Traditional RTT estimation methods usually depend on the cooperation of other networks and particular active or passive measurement platforms, whose global deployments are costly and difficult. Thus a new RTT estimation algorithm, ME algorithm, is introduced. It can estimate the RTT of two hosts communicating through border routers by using TCP CUBIC bulk flow data from those routhers without the use of extra facilities, which makes the RTT estimation in large-scale high-speed networks more effective. In addition, a simpler and more accurate algorithm — AE algorithm — is presented and used when the link has large bandwidth and low packet loss rate. The two proposed algorithms suit sampled flow data because only duration and total packet number of a TCP CUBIC bulk flow are inputs to their calculations. Experimental results show that both algorithms work excellently in real situations. Moreover, they have the potential to be adapted to other TCP versions with slight modification as their basic idea is independent of the TCP congestion control mechanism.