The effect of intermediate frequency magnetic fields or, very-low-frequency magnetic fields (VLFMF) on living biological cells was investigated using a highly sensitive mutagenesis assay method. A bacterial gene expression system for mutation repair (umu system) was used for the sensitive evaluation of damage in DNA molecules. Salmonella typhimurium TA1535 (pSK1002) were exposed to VLFMF (20 kHz and 600 µT) in a specially designed magnetic field loading chamber. The experiment results showed the possibility of applying the umu assay for sensitive and effective evaluation of damage in DNA molecules. No effects from exposure to 20 kHz and 600 µT magnetic fields in terms of damage in DNA molecules were observed.