In this paper, we propose an accurate calibration method for glassless stereoscopic systems. The method uses a lenticular lens on a general display. Glassless stereoscopic displays are currently used in many fields; however, accurately adjusting their physical display position is difficult because an accuracy of several microns or one hundredth of a degree is required, particularly given their larger display area. The proposed method enables a dynamic adjustment of the positions of images on the display to match various physical conditions in three-dimensional (3D) displays. In particular, compared with existing approaches, this avoids degradation of the image quality due to the image location on the screen while improving the image quality by local mapping. Moreover, it is shown to decrease the calibration time by performing simultaneous processing for each local area. As a result of the calibration, the offset jitter representing the crosstalk reduces from 14.946 to 8.645 mm. It is shown that high-quality 3D videos can be generated. Finally, we construct a stereoscopic viewing system using a high-resolution display and lenticular lens and produce high-quality 3D images with automatic calibration.