The effectiveness of Aeff enlarged positive dispersion fiber (EE-PDF) and hybrid amplification configuration with erbium-doped fiber amplifier (EDFA) and fiber Raman amplifier for reducing the fiber nonlinearity and improving the transmission performance in long distance 40 Gbit/s-based WDM transmission was investigated. We have confirmed that the use of EE-PDF in modified dispersion map for 40 Gbit/s transmission is quite effective to increase the transmissible distance and have successfully demonstrated 16 40 Gbit/s WDM transmission over 2000 km with proper dispersion management. We have also confirmed that the use of distributed Raman amplification is quite effective to extend the repeater spacing. By adding the optimum Raman amplification, almost the same transmission performance was obtained with a doubled repeater spacing in long distance 40 Gbit/s-based WDM transmission.