Transmission characteristics of a left-handed (LH) ferrite microstrip line are significantly affected by the nonuniform DC bias magnetic field in the ferrite substrate (internal magnetic field Hin) caused by the inhomogeneous demagnetizing effect because the strip conductors of these devices must be mounted at the edge of the ferrite substrate. Three dimensional analyses on the LH ferrite microstrip line are performed taking into account the nonuniform internal magnetic field Hin. The analytical results show that the nonuniform internal magnetic field under the strip conductor near the edge of the ferrite substrate is useful for spreading the frequency band of negative permeability and nonreciprocal operation, and for improvement of both the insertion and return losses of the LH ferrite microstrip line. Measured results of more than 20 dB isolation with 2.2 dB insertion loss and 1.33 GHz bandwidth are corresponding well to the analytical results.