An optical fiber biosensor using adsorption LB films has been investigated for the application to the glucose detection in blood. The adsorption LB film containing glucose oxidase (GOD: the enzyme in human body) was deposited on the core portion of optical fiber and the variation of absorption spectra due to the reactions of GOD, glucose, and hemoglobin was measured. The relatively high response time of 60 s and glucose sensitivity as low as 20 mg/dl were obtained. The results demonstrate that the optical fiber bio-sensor using adsorption LB film can be used as a glucose sensor.