An analysis is carried out about the reflection influence on the microwave attenuation measurement for moisture content determination. A new method taking into account the reflection influence is proposed and it is proved valid by the experiment results. Using this method, the density dependence of the attenuation is measured and the measured data can be fitted well by a straight line passing through the origin. Therefore, the attenuation per unit density and propagation distance is a function which depends only on the moisture content and the function is useful to the determination of the moisture content.