In formulating the motion constraint equation, we implicitly take it for granted that the spatial and temporal sampling intervals are very small. In real situations, since the intervals cannot be considered sufficiently small, an error will be introduced into the constraint equation and consequently the velocity estimate will be subject to an error due to inaccuracy of the constraint equation. We perform some experiments to analyze the effect of sampling interval on motion estimation. The understanding of experimental results will provide an insight into necessity and amount of image filtering prior to the application of motion estimation.