It is generally known that the autonomic nervous system regulates the pupil. In this study, we attempted to assess mental workload on the basis of the fluctuation rhythm in the pupil area. Controlling the respiration interval, we measured the pupil area during mental tasking for one minute. We simultaneously measured the respiration curve to monitor the respiration interval. We required the subject to perform two mental tasks. One was a mathematical division task, the difficulty of which was set to two, three, four, and five dividends. The other was a Sternberg memory search task, which had four work levels defined by the number of memory sets. In the Sternberg memory search, the number of memory set changed from five to eight. In such a way, we changed the mental workload induced by mental loading. As a result of calculating an autoregressive (AR) power spectrum, we could observe two peaks which corresponded to the blood pressure variation and respiratory sinus arrhythmia under a low workload. With an increased workload, the spectral peak related to the respiratory sinus arrhythmia disappeared. The ratio of the power at the low frequency band, from 0.05-0.15Hz, to the power at the respiration frequency band, from 0.35-0.4Hz, increased with the work level. In conclusion, the fluctuation of the pupil area is a promising means for the evaluation of mental workload or autonomic nervous function.

The aim of this study is to evaluate mental workload (MWL) quantitatively by HRV (Heart Rate Variability) measures. The electrocardiography and the respiration curve were recorded in five different epochs (1) during a rest condition and (2) during mental arithmetic tasks (addition). In the experiment, subjects added two numbers. The work levels (figures of the number in the addition) were set to one figure, two figures, three figures and four figures. The work level had effects on the mean percent correct, the number of answers and the mean processing time. The psychological evaluation on mental workload obtained by the method of paired comparison increased with the work level. Among the statistical HRV measures, the number of peak and trough waves could distinguish between the rest and the mental loading. However, mental workload for each work level was not evaluated quantitatively by the measure. The HRV measures were also calculated from the power spectrum estimated by the autoregressive (AR) model identification. The ratio of the low frequency power to the high frequency power increased linearly with the work level. In conclusion, the HRV measures obtained by the AR power spectrum analysis were found to be sensitive to changes of mental workload.