IEICE TRANSACTIONS on Electronics
Detection of Label-Free T4-DNA Molecules Using SPR Technique
Summary :
In this paper, we propose a new method for detecting label-free T4-DNA molecules quantitatively using a surface plasmon resonance (SPR) technique on a gold thin film. We used a solution that dissolved T4-DNA molecules in pure water, and examined the relationship between DNA concentration change and SPR angle change in the solution. As a result, it was confirmed that the SPR angle change increased with increasing DNA concentration change. Therefore, it was feasible to detect the DNA concentration change using the SPR technique. Furthermore, to examine and detect a single or a few DNA molecule, we tried to fabricate an SPR chip in which SPR area is narrowed so that it has the same effect as focusing the beam. To narrow the SPR area, we decreased the area of gold thin film in this chip, and, to reflect light from only the area of gold thin film, the area without a gold thin film was micromachined to increase its unevenness for the reduction of light reflection. By the above-mentioned method, we examined the possibility of detecting a label-free DNA molecule using the SPR technique.
- Publication
- IEICE TRANSACTIONS on Electronics Vol.E90-C No.1 pp.110-115
- Publication Date
- 2007/01/01
- Publicized
- Online ISSN
- 1745-1353
- DOI
- 10.1093/ietele/e90-c.1.110
- Type of Manuscript
- Special Section PAPER (Special Section on Microoptomechatronics)
- Category
- Plasmonics and Nanophotonics
Authors
Keyword
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Hiroki OKUNO, Ayami NISHIOKA, Maho HOSOGI, Fumikazu OOHIRA, Gen HASHIGUCHI, "Detection of Label-Free T4-DNA Molecules Using SPR Technique" in IEICE TRANSACTIONS on Electronics,
vol. E90-C, no. 1, pp. 110-115, January 2007, doi: 10.1093/ietele/e90-c.1.110.
Abstract: In this paper, we propose a new method for detecting label-free T4-DNA molecules quantitatively using a surface plasmon resonance (SPR) technique on a gold thin film. We used a solution that dissolved T4-DNA molecules in pure water, and examined the relationship between DNA concentration change and SPR angle change in the solution. As a result, it was confirmed that the SPR angle change increased with increasing DNA concentration change. Therefore, it was feasible to detect the DNA concentration change using the SPR technique. Furthermore, to examine and detect a single or a few DNA molecule, we tried to fabricate an SPR chip in which SPR area is narrowed so that it has the same effect as focusing the beam. To narrow the SPR area, we decreased the area of gold thin film in this chip, and, to reflect light from only the area of gold thin film, the area without a gold thin film was micromachined to increase its unevenness for the reduction of light reflection. By the above-mentioned method, we examined the possibility of detecting a label-free DNA molecule using the SPR technique.
URL: https://global.ieice.org/en_transactions/electronics/10.1093/ietele/e90-c.1.110/_p
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@ARTICLE{e90-c_1_110,
author={Hiroki OKUNO, Ayami NISHIOKA, Maho HOSOGI, Fumikazu OOHIRA, Gen HASHIGUCHI, },
journal={IEICE TRANSACTIONS on Electronics},
title={Detection of Label-Free T4-DNA Molecules Using SPR Technique},
year={2007},
volume={E90-C},
number={1},
pages={110-115},
abstract={In this paper, we propose a new method for detecting label-free T4-DNA molecules quantitatively using a surface plasmon resonance (SPR) technique on a gold thin film. We used a solution that dissolved T4-DNA molecules in pure water, and examined the relationship between DNA concentration change and SPR angle change in the solution. As a result, it was confirmed that the SPR angle change increased with increasing DNA concentration change. Therefore, it was feasible to detect the DNA concentration change using the SPR technique. Furthermore, to examine and detect a single or a few DNA molecule, we tried to fabricate an SPR chip in which SPR area is narrowed so that it has the same effect as focusing the beam. To narrow the SPR area, we decreased the area of gold thin film in this chip, and, to reflect light from only the area of gold thin film, the area without a gold thin film was micromachined to increase its unevenness for the reduction of light reflection. By the above-mentioned method, we examined the possibility of detecting a label-free DNA molecule using the SPR technique.},
keywords={},
doi={10.1093/ietele/e90-c.1.110},
ISSN={1745-1353},
month={January},}
Copy
TY - JOUR
TI - Detection of Label-Free T4-DNA Molecules Using SPR Technique
T2 - IEICE TRANSACTIONS on Electronics
SP - 110
EP - 115
AU - Hiroki OKUNO
AU - Ayami NISHIOKA
AU - Maho HOSOGI
AU - Fumikazu OOHIRA
AU - Gen HASHIGUCHI
PY - 2007
DO - 10.1093/ietele/e90-c.1.110
JO - IEICE TRANSACTIONS on Electronics
SN - 1745-1353
VL - E90-C
IS - 1
JA - IEICE TRANSACTIONS on Electronics
Y1 - January 2007
AB - In this paper, we propose a new method for detecting label-free T4-DNA molecules quantitatively using a surface plasmon resonance (SPR) technique on a gold thin film. We used a solution that dissolved T4-DNA molecules in pure water, and examined the relationship between DNA concentration change and SPR angle change in the solution. As a result, it was confirmed that the SPR angle change increased with increasing DNA concentration change. Therefore, it was feasible to detect the DNA concentration change using the SPR technique. Furthermore, to examine and detect a single or a few DNA molecule, we tried to fabricate an SPR chip in which SPR area is narrowed so that it has the same effect as focusing the beam. To narrow the SPR area, we decreased the area of gold thin film in this chip, and, to reflect light from only the area of gold thin film, the area without a gold thin film was micromachined to increase its unevenness for the reduction of light reflection. By the above-mentioned method, we examined the possibility of detecting a label-free DNA molecule using the SPR technique.
ER -
English
