Formation of Black Membrane Using a Microfabricated Orifice
Summary :
A black membrane is a biological-membrane analogue, i.e. a phospholipid bilayer membrane, artificially formed on an orifice immersed in water. It is used to investigate the properties of the membrane itself and channels embedded therein. In this paper, microfabrication techniques are applied to fabricate the orifice, and a glass substrate is isotropically etched to define the orifice geometry. The periphery of the orifice was patterned with aminosilane to anchor the membrane. The remainder part was coated with fluorosilane to make the surface hydrophobic and to prevent adsorption of channel-forming molecules. We demonstrated experimentally that a stable and reproducible membrane is easily obtainable using the orifice.
- Publication
- IEICE TRANSACTIONS on Electronics Vol.E78-C No.2 pp.157-161
- Publication Date
- 1995/02/25
- Publicized
- Online ISSN
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- Type of Manuscript
- Special Section PAPER (Special Issue on Micromachines and Micro Electro Mechanical Systems)
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Masao WASHIZU, Seiichi SUZUKI, Osamu KUROSAWA, Hideaki KURAHASHI, Akira KATOH, "Formation of Black Membrane Using a Microfabricated Orifice" in IEICE TRANSACTIONS on Electronics,
vol. E78-C, no. 2, pp. 157-161, February 1995, doi: .
Abstract: A black membrane is a biological-membrane analogue, i.e. a phospholipid bilayer membrane, artificially formed on an orifice immersed in water. It is used to investigate the properties of the membrane itself and channels embedded therein. In this paper, microfabrication techniques are applied to fabricate the orifice, and a glass substrate is isotropically etched to define the orifice geometry. The periphery of the orifice was patterned with aminosilane to anchor the membrane. The remainder part was coated with fluorosilane to make the surface hydrophobic and to prevent adsorption of channel-forming molecules. We demonstrated experimentally that a stable and reproducible membrane is easily obtainable using the orifice.
URL: https://global.ieice.org/en_transactions/electronics/10.1587/e78-c_2_157/_p
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@ARTICLE{e78-c_2_157,
author={Masao WASHIZU, Seiichi SUZUKI, Osamu KUROSAWA, Hideaki KURAHASHI, Akira KATOH, },
journal={IEICE TRANSACTIONS on Electronics},
title={Formation of Black Membrane Using a Microfabricated Orifice},
year={1995},
volume={E78-C},
number={2},
pages={157-161},
abstract={A black membrane is a biological-membrane analogue, i.e. a phospholipid bilayer membrane, artificially formed on an orifice immersed in water. It is used to investigate the properties of the membrane itself and channels embedded therein. In this paper, microfabrication techniques are applied to fabricate the orifice, and a glass substrate is isotropically etched to define the orifice geometry. The periphery of the orifice was patterned with aminosilane to anchor the membrane. The remainder part was coated with fluorosilane to make the surface hydrophobic and to prevent adsorption of channel-forming molecules. We demonstrated experimentally that a stable and reproducible membrane is easily obtainable using the orifice.},
keywords={},
doi={},
ISSN={},
month={February},}
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TY - JOUR
TI - Formation of Black Membrane Using a Microfabricated Orifice
T2 - IEICE TRANSACTIONS on Electronics
SP - 157
EP - 161
AU - Masao WASHIZU
AU - Seiichi SUZUKI
AU - Osamu KUROSAWA
AU - Hideaki KURAHASHI
AU - Akira KATOH
PY - 1995
DO -
JO - IEICE TRANSACTIONS on Electronics
SN -
VL - E78-C
IS - 2
JA - IEICE TRANSACTIONS on Electronics
Y1 - February 1995
AB - A black membrane is a biological-membrane analogue, i.e. a phospholipid bilayer membrane, artificially formed on an orifice immersed in water. It is used to investigate the properties of the membrane itself and channels embedded therein. In this paper, microfabrication techniques are applied to fabricate the orifice, and a glass substrate is isotropically etched to define the orifice geometry. The periphery of the orifice was patterned with aminosilane to anchor the membrane. The remainder part was coated with fluorosilane to make the surface hydrophobic and to prevent adsorption of channel-forming molecules. We demonstrated experimentally that a stable and reproducible membrane is easily obtainable using the orifice.
ER -
English
