Hepatocyte Spheroids Underlayered with Nonparenchymal Cells for Biomedical Applications

Yuichi NAKASONE; Masashi YAMAMOTO; Tetsuya TATEISHI; Hidenori OTSUKA

doi:10.1587/transele.E94.C.176

Hepatocyte Spheroids Underlayered with Nonparenchymal Cells for Biomedical Applications

Yuichi NAKASONE, Masashi YAMAMOTO, Tetsuya TATEISHI, Hidenori OTSUKA

Full Text Views

0

Cite this

Summary :

A two-dimensional microarray of ten thousand (100100) hepatocyte hetero-spheroids, underlaid with non-parenchymal cells, was successfully constructed with a 100-µm spacing on micro-fabricated glass substrates that were coated with poly(ethylene glycol) (PEG). Co-cultivation of hepatocytes with endothelial cells was essential to stabilize hepatocyte viability and liver-specific functions, allowing us to obtain hepatocyte spheroids with a diameter of 100-µm, functioning as a miniaturized liver to secrete albumin for at least 1 month. The most important feature of this study is that these substrates are defined to provide an unprecedented control of substrate properties for modulating cell behavior, employing both surface engineering and synthetic polymer chemistry. The spheroid array constructed here is highly useful as a platform of tissue and cell-based biosensors (TBB and CBB), detecting a wide variety of clinically, pharmacologically, and toxicologically active compounds through a cellular physiological response.

Publication: IEICE TRANSACTIONS on Electronics Vol.E94-C No.2 pp.176-180

Publication Date: 2011/02/01

Publicized

Online ISSN: 1745-1353

DOI: 10.1587/transele.E94.C.176

Type of Manuscript: Special Section PAPER (Special Section on Recent Progress in Molecular and Organic Devices)

Category

Cite this

Copy

Yuichi NAKASONE, Masashi YAMAMOTO, Tetsuya TATEISHI, Hidenori OTSUKA, "Hepatocyte Spheroids Underlayered with Nonparenchymal Cells for Biomedical Applications" in IEICE TRANSACTIONS on Electronics, vol. E94-C, no. 2, pp. 176-180, February 2011, doi: 10.1587/transele.E94.C.176.
Abstract: A two-dimensional microarray of ten thousand (100100) hepatocyte hetero-spheroids, underlaid with non-parenchymal cells, was successfully constructed with a 100-µm spacing on micro-fabricated glass substrates that were coated with poly(ethylene glycol) (PEG). Co-cultivation of hepatocytes with endothelial cells was essential to stabilize hepatocyte viability and liver-specific functions, allowing us to obtain hepatocyte spheroids with a diameter of 100-µm, functioning as a miniaturized liver to secrete albumin for at least 1 month. The most important feature of this study is that these substrates are defined to provide an unprecedented control of substrate properties for modulating cell behavior, employing both surface engineering and synthetic polymer chemistry. The spheroid array constructed here is highly useful as a platform of tissue and cell-based biosensors (TBB and CBB), detecting a wide variety of clinically, pharmacologically, and toxicologically active compounds through a cellular physiological response.
URL: https://global.ieice.org/en_transactions/electronics/10.1587/transele.E94.C.176/_p

Copy

@ARTICLE{e94-c_2_176,
author={Yuichi NAKASONE, Masashi YAMAMOTO, Tetsuya TATEISHI, Hidenori OTSUKA, },
journal={IEICE TRANSACTIONS on Electronics},
title={Hepatocyte Spheroids Underlayered with Nonparenchymal Cells for Biomedical Applications},
year={2011},
volume={E94-C},
number={2},
pages={176-180},
abstract={A two-dimensional microarray of ten thousand (100100) hepatocyte hetero-spheroids, underlaid with non-parenchymal cells, was successfully constructed with a 100-µm spacing on micro-fabricated glass substrates that were coated with poly(ethylene glycol) (PEG). Co-cultivation of hepatocytes with endothelial cells was essential to stabilize hepatocyte viability and liver-specific functions, allowing us to obtain hepatocyte spheroids with a diameter of 100-µm, functioning as a miniaturized liver to secrete albumin for at least 1 month. The most important feature of this study is that these substrates are defined to provide an unprecedented control of substrate properties for modulating cell behavior, employing both surface engineering and synthetic polymer chemistry. The spheroid array constructed here is highly useful as a platform of tissue and cell-based biosensors (TBB and CBB), detecting a wide variety of clinically, pharmacologically, and toxicologically active compounds through a cellular physiological response.},
keywords={},
doi={10.1587/transele.E94.C.176},
ISSN={1745-1353},
month={February},}

Copy

TY - JOUR
TI - Hepatocyte Spheroids Underlayered with Nonparenchymal Cells for Biomedical Applications
T2 - IEICE TRANSACTIONS on Electronics
SP - 176
EP - 180
AU - Yuichi NAKASONE
AU - Masashi YAMAMOTO
AU - Tetsuya TATEISHI
AU - Hidenori OTSUKA
PY - 2011
DO - 10.1587/transele.E94.C.176
JO - IEICE TRANSACTIONS on Electronics
SN - 1745-1353
VL - E94-C
IS - 2
JA - IEICE TRANSACTIONS on Electronics
Y1 - February 2011
AB - A two-dimensional microarray of ten thousand (100100) hepatocyte hetero-spheroids, underlaid with non-parenchymal cells, was successfully constructed with a 100-µm spacing on micro-fabricated glass substrates that were coated with poly(ethylene glycol) (PEG). Co-cultivation of hepatocytes with endothelial cells was essential to stabilize hepatocyte viability and liver-specific functions, allowing us to obtain hepatocyte spheroids with a diameter of 100-µm, functioning as a miniaturized liver to secrete albumin for at least 1 month. The most important feature of this study is that these substrates are defined to provide an unprecedented control of substrate properties for modulating cell behavior, employing both surface engineering and synthetic polymer chemistry. The spheroid array constructed here is highly useful as a platform of tissue and cell-based biosensors (TBB and CBB), detecting a wide variety of clinically, pharmacologically, and toxicologically active compounds through a cellular physiological response.
ER -