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Hidenori OTSUKA, Masako NAGAMURA, Akie KANEKO, Koichi KUTSUZAWA, Toshiya SAKATA, "Label-Free and Noninvasive Monitoring of Cell Differentiation on Spheroid Microarray" in IEICE TRANSACTIONS on Electronics,
vol. E96-C, no. 3, pp. 353-357, March 2013, doi: 10.1587/transele.E96.C.353.
Abstract: A two-dimensional microarray of ten thousand (100100) chondrocyte-spheroids was successfully constructed with a 100-µm spacing on a micropatterned gold electrodes that were coated with poly(ethylene glycol) (PEG) hydrogels. The PEGylated surface as a cytophobic region was regulated by controlling the gel structure through photolithography. In this way, a PEG hydrogel was modulated enough to inhibit outgrowth of chondrocytes from cell adhering region in the horizontal direction. These structural control of PEG hydrogel was critical for inducing formation of three-dimensional chondrocyte condensations (spheroids) within 24 hours. We report noninvasive monitoring of the cellular functional change at the cell membrane using a chondrocyte-based field effect transistor (FET), which is based on detection of extracellular potential change induced as a result of the interaction between extracellular matrix (ECM) protein secreted from spheroid and substrate at the cell membrane. The interface potential change at the cell membrane/gate insulator interface can be monitored during the uptake of substrate without any labeling materials. Our findings on the time course of the interface potential would provide important information to understand the uptake kinetics for cellular differentiation.
URL: https://global.ieice.org/en_transactions/electronics/10.1587/transele.E96.C.353/_p
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@ARTICLE{e96-c_3_353,
author={Hidenori OTSUKA, Masako NAGAMURA, Akie KANEKO, Koichi KUTSUZAWA, Toshiya SAKATA, },
journal={IEICE TRANSACTIONS on Electronics},
title={Label-Free and Noninvasive Monitoring of Cell Differentiation on Spheroid Microarray},
year={2013},
volume={E96-C},
number={3},
pages={353-357},
abstract={A two-dimensional microarray of ten thousand (100100) chondrocyte-spheroids was successfully constructed with a 100-µm spacing on a micropatterned gold electrodes that were coated with poly(ethylene glycol) (PEG) hydrogels. The PEGylated surface as a cytophobic region was regulated by controlling the gel structure through photolithography. In this way, a PEG hydrogel was modulated enough to inhibit outgrowth of chondrocytes from cell adhering region in the horizontal direction. These structural control of PEG hydrogel was critical for inducing formation of three-dimensional chondrocyte condensations (spheroids) within 24 hours. We report noninvasive monitoring of the cellular functional change at the cell membrane using a chondrocyte-based field effect transistor (FET), which is based on detection of extracellular potential change induced as a result of the interaction between extracellular matrix (ECM) protein secreted from spheroid and substrate at the cell membrane. The interface potential change at the cell membrane/gate insulator interface can be monitored during the uptake of substrate without any labeling materials. Our findings on the time course of the interface potential would provide important information to understand the uptake kinetics for cellular differentiation.},
keywords={},
doi={10.1587/transele.E96.C.353},
ISSN={1745-1353},
month={March},}
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TY - JOUR
TI - Label-Free and Noninvasive Monitoring of Cell Differentiation on Spheroid Microarray
T2 - IEICE TRANSACTIONS on Electronics
SP - 353
EP - 357
AU - Hidenori OTSUKA
AU - Masako NAGAMURA
AU - Akie KANEKO
AU - Koichi KUTSUZAWA
AU - Toshiya SAKATA
PY - 2013
DO - 10.1587/transele.E96.C.353
JO - IEICE TRANSACTIONS on Electronics
SN - 1745-1353
VL - E96-C
IS - 3
JA - IEICE TRANSACTIONS on Electronics
Y1 - March 2013
AB - A two-dimensional microarray of ten thousand (100100) chondrocyte-spheroids was successfully constructed with a 100-µm spacing on a micropatterned gold electrodes that were coated with poly(ethylene glycol) (PEG) hydrogels. The PEGylated surface as a cytophobic region was regulated by controlling the gel structure through photolithography. In this way, a PEG hydrogel was modulated enough to inhibit outgrowth of chondrocytes from cell adhering region in the horizontal direction. These structural control of PEG hydrogel was critical for inducing formation of three-dimensional chondrocyte condensations (spheroids) within 24 hours. We report noninvasive monitoring of the cellular functional change at the cell membrane using a chondrocyte-based field effect transistor (FET), which is based on detection of extracellular potential change induced as a result of the interaction between extracellular matrix (ECM) protein secreted from spheroid and substrate at the cell membrane. The interface potential change at the cell membrane/gate insulator interface can be monitored during the uptake of substrate without any labeling materials. Our findings on the time course of the interface potential would provide important information to understand the uptake kinetics for cellular differentiation.
ER -
English
