We describe the preparation of an α-phenyl-4'-(diphenylamino)stilbene (TPA) single crystal and the evaluation of its hole transport property. Based on the characterization using optical microscopy, polarizing microscopy, and X-ray diffraction, a large-scale TPA single crystal of dimensions 7.0×0.9×0.8mm is successfully synthesized using a solution method based on the solubility and supersolubility curves of the TPA. Notably, the current in the long-axis direction is larger than those in the short-axis and thickness directions (i(long) > i(short) > i(thickness)), which reveals the anisotropic charge transfer of the TPA single crystal. The observed anisotropic conductivity is well explained by the orientation of the triphenylamine unit in the TPA single crystal. Furthermore, the activation energy of the long-axis direction in the TPA single crystal is lower than that of the short-axis in TPA and all the axes in the α-phenyl-4'-[bis(4-methylphenyl)amino]stilbene single crystal reported in our previous study.