A novel sensing system for glucose in aqueous solution based on cataluminescence(CTL) is proposed. CTL is a kind of chemiluminescence emitted in a course of catalytic oxidation of combustible substances. A sensing system consisting of a CTL-based chemical-sensor made of the γ-Al2O3 catalyst activated with Tb and an ultrasonic nebulizer is developed. CTL is emitted by injection of air containing mist of a glucose solution prepared by the nebulizer on the catalyst. The CTL intensity measured by a photomultiplier is reproducible for the repeated injection of the mist, and the system can measure glucose concentration in a range of 1-200 mg/dl.