A rapid testing is required in clinical laboratory. A new method for rapid bacterial testing is proposed by size distribution measurement with laser light scattering. Bacterial sample is suspended in a saline solution and the scattering pattern is measured from 5 to 90 degrees with 5-degree intervals. The size distribution function is calculated from the light scattering pattern by the inversion technique based on the modified Rayleigh-Debye approximation. The difference in bacterial species and the influences of drug are investigated. The following results are obtained; (1) size distribution curve reflects the morphological characteristics of bacteria, and bacterial species are discriminated roughly by two characteristic parameters (mean size and dispersion width) obtained from the curve; (2) size distribution pattern changes in a short time (within one hour) indicating the effects of drug. It is useful for antibiotics susceptibility testing.

Caenorhabditis elegans during feeding gives good moving biological images",in which motions of several pulsing organs are superposed on its head swing. A powerful method to extract dynamic features is presented. First step is to use a variance picture VAG4 in order to pick up active pixel coordinates of concerned moving objects. Superiority of VAG4 over usual variance picture VAG2 is shown quantitatively by a model of moving particles. Pulsing areas of C. elegans, are exhibited more clearly in VAG4 than VAG2. Second step is use of a new subtraction method to extract main frequency bands. FFT spectra are averaged in active positions where VAG4 is above threshold THVR in the square with 88 pixels (ONA). The power spectra averaged in the enlarged squares (ELA) are subtracted from those in ONA, in which ELA includes ONA in its centre position. Large peak bands emerge in the subtracted power spectra. The subtraction eliminates the effect of head swing by spatial averagings in ELA. This new emphasizing method is compared to another subtraction method. The characteristic frequency of periodical moving organs coincides well with the values observed by other research groups and our visual estimation of replayed VTR images. Thus the proposed extraction method is verified to work well in double superposed motions.