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IEICE TRANSACTIONS on transactions

Rapid Bacterial Testing Method by Size Distribution Measurement with Laser Light Scattering

Hajime HASHIMOTO, Koji KAKIHARA, Hidetoshi MIIKE, Yoshio EBINA

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Summary :

A rapid testing is required in clinical laboratory. A new method for rapid bacterial testing is proposed by size distribution measurement with laser light scattering. Bacterial sample is suspended in a saline solution and the scattering pattern is measured from 5 to 90 degrees with 5-degree intervals. The size distribution function is calculated from the light scattering pattern by the inversion technique based on the modified Rayleigh-Debye approximation. The difference in bacterial species and the influences of drug are investigated. The following results are obtained; (1) size distribution curve reflects the morphological characteristics of bacteria, and bacterial species are discriminated roughly by two characteristic parameters (mean size and dispersion width) obtained from the curve; (2) size distribution pattern changes in a short time (within one hour) indicating the effects of drug. It is useful for antibiotics susceptibility testing.

Publication
IEICE TRANSACTIONS on transactions Vol.E68-E No.5 pp.304-308
Publication Date
1985/05/25
Publicized
Online ISSN
DOI
Type of Manuscript
PAPER
Category
Optical and Quantum Electronics

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