In the present paper, procedures for extracting nucleoli from leukocyte images are proposed. Main processed procedures consist of the filtering operation in the frequency domain, the edge preserving smoothing, the extraction of the nuclear region, the edge detection and the decision of nucleolar regions. By applying proposed procedures, nucleoli can be extracted easily and precisely from the nucleus if we can clearly see nucleoli with human eyes. We were able to achieve the correct discrimination of 82 percent of nucleoli in immature leukocytes.