In this letter, a new output feedback tracking control using a fuzzy disturbance observer (FDO) is proposed and its application to control of a nonlinear system in the presence of the internal parameter perturbation and external disturbance is presented. An FDO using a filtered signal is developed and the high gain observer (HGO) is employed to implement the output feedback tracking control. It is shown in a rigorous manner that all the errors involved can be kept arbitrarily small. Finally, the effectiveness and the feasibility of the suggested method is demonstrated by computer simulation.