This paper presents a design methodology for positioning sub-platform from the viewpoint of positioning for smartphone-based location-based services (LBS). To achieve this, we analyze a mechanism of positioning error generation including principles of positioning sub-systems and structure of smartphones. Specifically, we carry out the experiments of smartphone positioning performance evaluation by the smartphone basic API (Application Programming Interface) and by the wireless LAN in various environments. Then, we describe the importance of considering three layers as follows: 1) the lower layer that caused by positioning sub-systems, e.g., GPS, wireless LAN, mobile base stations, and so on; 2) the middle layer that caused by functions provided from the platform such as Android and iOS; 3) the upper layer that caused by operation algorithm of applications on the platform.