IEICE TRANSACTIONS on Electronics
Sulfate Binding Protein Modified Electrode as a Chemical Sensor
Summary :
A novel chemical sensor for sulfate detection was proposed in this study, utilizing sulfate binding protein (SBP) derived from Escherichia coli as sulfate recognition element. Purified SBP was immobilized on a gold electrode modified with cysteamine and glutaraldehyde. In this study the surface potential change of the SBP modified electrode to sulfate and various ions were investigated. In order to evaluate nonspecific interaction with ionic species, proteins with various isoelectric point were immobilized on the surface of gold electrode and response to ions were measured and compared to sulfate binding protein modified electrode. We made clear that the protein modified electrode shows the potential change to ions and these potential change was effected by the isoelectric point of the protein molecule, and BSA, whose isoelectric point is closest to that of SBP, showed the similar response to ions except sulfate. With use BSA modified electrode as a reference electrode, this sensing system showed selective response to sulfate, probably because of the selective binding sulfate by SBP. This potential change difference between the SBP modified electrode and the BSA modified electrode depended on the concentration of sulfate with in the range of 5 - 150 mM.
- Publication
- IEICE TRANSACTIONS on Electronics Vol.E83-C No.7 pp.1035-1039
- Publication Date
- 2000/07/25
- Publicized
- Online ISSN
- DOI
- Type of Manuscript
- Special Section PAPER (Special Issue on Organic Molecular Electronics for the 21st Century)
- Category
- Sensor
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Keyword
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Izumi KUBO, Hidenori NAGAI, "Sulfate Binding Protein Modified Electrode as a Chemical Sensor" in IEICE TRANSACTIONS on Electronics,
vol. E83-C, no. 7, pp. 1035-1039, July 2000, doi: .
Abstract: A novel chemical sensor for sulfate detection was proposed in this study, utilizing sulfate binding protein (SBP) derived from Escherichia coli as sulfate recognition element. Purified SBP was immobilized on a gold electrode modified with cysteamine and glutaraldehyde. In this study the surface potential change of the SBP modified electrode to sulfate and various ions were investigated. In order to evaluate nonspecific interaction with ionic species, proteins with various isoelectric point were immobilized on the surface of gold electrode and response to ions were measured and compared to sulfate binding protein modified electrode. We made clear that the protein modified electrode shows the potential change to ions and these potential change was effected by the isoelectric point of the protein molecule, and BSA, whose isoelectric point is closest to that of SBP, showed the similar response to ions except sulfate. With use BSA modified electrode as a reference electrode, this sensing system showed selective response to sulfate, probably because of the selective binding sulfate by SBP. This potential change difference between the SBP modified electrode and the BSA modified electrode depended on the concentration of sulfate with in the range of 5 - 150 mM.
URL: https://global.ieice.org/en_transactions/electronics/10.1587/e83-c_7_1035/_p
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@ARTICLE{e83-c_7_1035,
author={Izumi KUBO, Hidenori NAGAI, },
journal={IEICE TRANSACTIONS on Electronics},
title={Sulfate Binding Protein Modified Electrode as a Chemical Sensor},
year={2000},
volume={E83-C},
number={7},
pages={1035-1039},
abstract={A novel chemical sensor for sulfate detection was proposed in this study, utilizing sulfate binding protein (SBP) derived from Escherichia coli as sulfate recognition element. Purified SBP was immobilized on a gold electrode modified with cysteamine and glutaraldehyde. In this study the surface potential change of the SBP modified electrode to sulfate and various ions were investigated. In order to evaluate nonspecific interaction with ionic species, proteins with various isoelectric point were immobilized on the surface of gold electrode and response to ions were measured and compared to sulfate binding protein modified electrode. We made clear that the protein modified electrode shows the potential change to ions and these potential change was effected by the isoelectric point of the protein molecule, and BSA, whose isoelectric point is closest to that of SBP, showed the similar response to ions except sulfate. With use BSA modified electrode as a reference electrode, this sensing system showed selective response to sulfate, probably because of the selective binding sulfate by SBP. This potential change difference between the SBP modified electrode and the BSA modified electrode depended on the concentration of sulfate with in the range of 5 - 150 mM.},
keywords={},
doi={},
ISSN={},
month={July},}
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TY - JOUR
TI - Sulfate Binding Protein Modified Electrode as a Chemical Sensor
T2 - IEICE TRANSACTIONS on Electronics
SP - 1035
EP - 1039
AU - Izumi KUBO
AU - Hidenori NAGAI
PY - 2000
DO -
JO - IEICE TRANSACTIONS on Electronics
SN -
VL - E83-C
IS - 7
JA - IEICE TRANSACTIONS on Electronics
Y1 - July 2000
AB - A novel chemical sensor for sulfate detection was proposed in this study, utilizing sulfate binding protein (SBP) derived from Escherichia coli as sulfate recognition element. Purified SBP was immobilized on a gold electrode modified with cysteamine and glutaraldehyde. In this study the surface potential change of the SBP modified electrode to sulfate and various ions were investigated. In order to evaluate nonspecific interaction with ionic species, proteins with various isoelectric point were immobilized on the surface of gold electrode and response to ions were measured and compared to sulfate binding protein modified electrode. We made clear that the protein modified electrode shows the potential change to ions and these potential change was effected by the isoelectric point of the protein molecule, and BSA, whose isoelectric point is closest to that of SBP, showed the similar response to ions except sulfate. With use BSA modified electrode as a reference electrode, this sensing system showed selective response to sulfate, probably because of the selective binding sulfate by SBP. This potential change difference between the SBP modified electrode and the BSA modified electrode depended on the concentration of sulfate with in the range of 5 - 150 mM.
ER -
English
