Development of Biological Micro Reactor Array System

Etsuo SHINOHARA; Seiji KONDO; Kouki AKAHORI; Kohichi TASHIRO; Shuichi SHOJI

Development of Biological Micro Reactor Array System

Etsuo SHINOHARA, Seiji KONDO, Kouki AKAHORI, Kohichi TASHIRO, Shuichi SHOJI

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A micro reactor array for biochemical or biomedical use was developed. Conceptof this development is to get as much as biological data at the same time. Ninety-six micro reaction wells, volume of each well was 1.5 µl, were integrated in the array. The micro reactor array was fabricated on 1 mm thick silicon wafer and twelve pairs of a temperature sensor and a heater were formed on the backside. A tiny transparent window for optical measurement was formed at the center of bottom wall on each well. Several temperature gradients were applied to the array by means of few heaters and compared with simulation results to optimize the parameters. Finally, performance of the array was evaluated by basic DNA reaction. Advantages of the array system are the fast thermal response due to the small heat capacity and easy to make several reaction conditions in parallel.

Publication: IEICE TRANSACTIONS on Electronics Vol.E84-C No.12 pp.1807-1813

Publication Date: 2001/12/01

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Type of Manuscript: Special Section PAPER (Special Issue on Integrated Systems with New Concepts)

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Etsuo SHINOHARA, Seiji KONDO, Kouki AKAHORI, Kohichi TASHIRO, Shuichi SHOJI, "Development of Biological Micro Reactor Array System" in IEICE TRANSACTIONS on Electronics, vol. E84-C, no. 12, pp. 1807-1813, December 2001, doi: .
Abstract: A micro reactor array for biochemical or biomedical use was developed. Conceptof this development is to get as much as biological data at the same time. Ninety-six micro reaction wells, volume of each well was 1.5 µl, were integrated in the array. The micro reactor array was fabricated on 1 mm thick silicon wafer and twelve pairs of a temperature sensor and a heater were formed on the backside. A tiny transparent window for optical measurement was formed at the center of bottom wall on each well. Several temperature gradients were applied to the array by means of few heaters and compared with simulation results to optimize the parameters. Finally, performance of the array was evaluated by basic DNA reaction. Advantages of the array system are the fast thermal response due to the small heat capacity and easy to make several reaction conditions in parallel.
URL: https://global.ieice.org/en_transactions/electronics/10.1587/e84-c_12_1807/_p

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@ARTICLE{e84-c_12_1807,
author={Etsuo SHINOHARA, Seiji KONDO, Kouki AKAHORI, Kohichi TASHIRO, Shuichi SHOJI, },
journal={IEICE TRANSACTIONS on Electronics},
title={Development of Biological Micro Reactor Array System},
year={2001},
volume={E84-C},
number={12},
pages={1807-1813},
abstract={A micro reactor array for biochemical or biomedical use was developed. Conceptof this development is to get as much as biological data at the same time. Ninety-six micro reaction wells, volume of each well was 1.5 µl, were integrated in the array. The micro reactor array was fabricated on 1 mm thick silicon wafer and twelve pairs of a temperature sensor and a heater were formed on the backside. A tiny transparent window for optical measurement was formed at the center of bottom wall on each well. Several temperature gradients were applied to the array by means of few heaters and compared with simulation results to optimize the parameters. Finally, performance of the array was evaluated by basic DNA reaction. Advantages of the array system are the fast thermal response due to the small heat capacity and easy to make several reaction conditions in parallel.},
keywords={},
doi={},
ISSN={},
month={December},}

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TY - JOUR
TI - Development of Biological Micro Reactor Array System
T2 - IEICE TRANSACTIONS on Electronics
SP - 1807
EP - 1813
AU - Etsuo SHINOHARA
AU - Seiji KONDO
AU - Kouki AKAHORI
AU - Kohichi TASHIRO
AU - Shuichi SHOJI
PY - 2001
DO -
JO - IEICE TRANSACTIONS on Electronics
SN -
VL - E84-C
IS - 12
JA - IEICE TRANSACTIONS on Electronics
Y1 - December 2001
AB - A micro reactor array for biochemical or biomedical use was developed. Conceptof this development is to get as much as biological data at the same time. Ninety-six micro reaction wells, volume of each well was 1.5 µl, were integrated in the array. The micro reactor array was fabricated on 1 mm thick silicon wafer and twelve pairs of a temperature sensor and a heater were formed on the backside. A tiny transparent window for optical measurement was formed at the center of bottom wall on each well. Several temperature gradients were applied to the array by means of few heaters and compared with simulation results to optimize the parameters. Finally, performance of the array was evaluated by basic DNA reaction. Advantages of the array system are the fast thermal response due to the small heat capacity and easy to make several reaction conditions in parallel.
ER -