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In Situ Observation of Reduction Behavior of Cytochrome c Adsorbed on Glass Surface by Slab Optical Waveguide Spectroscopy

Naoki MATSUDA, Hirotaka OKABE, Masaki FUJII, Masayoshi MATSUI, Yusuke AYATO, Akiko TAKATSU, Kenji KATO

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Summary :

In situ observation of the adsorption process and the states of cytochrome c on glass/solution interfaces, and the functionality of the reduction reaction of adsorbed cytochorome c were performed by using slab optical waveguide (SOWG) spectroscopy. The peak position of the absorption band of cytochorome c adsorbed on a bare glass surface was almost the same as that of that in solution. The cytochorome c adsorbed on glass/solution interface was reduced by sodium dithionite solution. The adsorbed cytochorome c was still maintained its functionality after immobilization.

Publication
IEICE TRANSACTIONS on Electronics Vol.E94-C No.2 pp.170-175
Publication Date
2011/02/01
Publicized
Online ISSN
1745-1353
DOI
10.1587/transele.E94.C.170
Type of Manuscript
Special Section PAPER (Special Section on Recent Progress in Molecular and Organic Devices)
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