In situ Observation of Direct Electron Transfer Reaction of Cytochrome c Immobilized on ITO Electrode Modified with 10-carboxydecylphosphonic Acid by Slab Optical Waveguide Spectroscopy and Cyclic Voltammetry
Summary :
To immobilize cytochrome c (cyt. c) on ITO electrode with keeping its direct electron transfer (DET) activity, 10-carboxydecylphosphonic acid (10-CDPA) self-assembled monolayer (SAM) film was formed on ITO electrode. After 100 times washing process with exchanging phosphate buffer saline solution in the cell to fresh one, in situ slab optical waveguide (SOWG) absorption spectral measurement proved that about 80% of cyt. c immobilized on 10-CDPA modified ITO electrode was adsorbed on ITO electrode. Additionally SOWG spectral change of cyt. c between oxidized and reduced forms was observed with setting the ITO electrode potential at 0.3 and -0.3 V vs. Ag/AgCl, respectively showing DET reaction between cyt. c and ITO electrode occurred. About 30% of a monolayer coverage was estimated from the coulomb amount in the surface area of oxidation and reduction peaks on cyclic voltammetry (CV) data. CV peak current maintained 84% for ITO electrode modified with 10-CDPA SAM film after 60 min continuous scan with 0.1 V/sec from 0.3 and -0.3 V vs. Ag/AgCl.
- Publication
- IEICE TRANSACTIONS on Electronics Vol.E98-C No.2 pp.152-155
- Publication Date
- 2015/02/01
- Publicized
- Online ISSN
- 1745-1353
- DOI
- 10.1587/transele.E98.C.152
- Type of Manuscript
- BRIEF PAPER
- Category
Authors
Naoki MATSUDA
National Institute of Advanced Industrial Science and Technology (AIST)
Hirotaka OKABE
National Institute of Advanced Industrial Science and Technology (AIST)
Keyword
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Naoki MATSUDA, Hirotaka OKABE, " extit{In situ} Observation of Direct Electron Transfer Reaction of Cytochrome extit{c} Immobilized on ITO Electrode Modified with 10-carboxydecylphosphonic Acid by Slab Optical Waveguide Spectroscopy and Cyclic Voltammetry" in IEICE TRANSACTIONS on Electronics,
vol. E98-C, no. 2, pp. 152-155, February 2015, doi: 10.1587/transele.E98.C.152.
Abstract: To immobilize cytochrome c (cyt. c) on ITO electrode with keeping its direct electron transfer (DET) activity, 10-carboxydecylphosphonic acid (10-CDPA) self-assembled monolayer (SAM) film was formed on ITO electrode. After 100 times washing process with exchanging phosphate buffer saline solution in the cell to fresh one, in situ slab optical waveguide (SOWG) absorption spectral measurement proved that about 80% of cyt. c immobilized on 10-CDPA modified ITO electrode was adsorbed on ITO electrode. Additionally SOWG spectral change of cyt. c between oxidized and reduced forms was observed with setting the ITO electrode potential at 0.3 and -0.3 V vs. Ag/AgCl, respectively showing DET reaction between cyt. c and ITO electrode occurred. About 30% of a monolayer coverage was estimated from the coulomb amount in the surface area of oxidation and reduction peaks on cyclic voltammetry (CV) data. CV peak current maintained 84% for ITO electrode modified with 10-CDPA SAM film after 60 min continuous scan with 0.1 V/sec from 0.3 and -0.3 V vs. Ag/AgCl.
URL: https://global.ieice.org/en_transactions/electronics/10.1587/transele.E98.C.152/_p
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@ARTICLE{e98-c_2_152,
author={Naoki MATSUDA, Hirotaka OKABE, },
journal={IEICE TRANSACTIONS on Electronics},
title={ extit{In situ} Observation of Direct Electron Transfer Reaction of Cytochrome extit{c} Immobilized on ITO Electrode Modified with 10-carboxydecylphosphonic Acid by Slab Optical Waveguide Spectroscopy and Cyclic Voltammetry},
year={2015},
volume={E98-C},
number={2},
pages={152-155},
abstract={To immobilize cytochrome c (cyt. c) on ITO electrode with keeping its direct electron transfer (DET) activity, 10-carboxydecylphosphonic acid (10-CDPA) self-assembled monolayer (SAM) film was formed on ITO electrode. After 100 times washing process with exchanging phosphate buffer saline solution in the cell to fresh one, in situ slab optical waveguide (SOWG) absorption spectral measurement proved that about 80% of cyt. c immobilized on 10-CDPA modified ITO electrode was adsorbed on ITO electrode. Additionally SOWG spectral change of cyt. c between oxidized and reduced forms was observed with setting the ITO electrode potential at 0.3 and -0.3 V vs. Ag/AgCl, respectively showing DET reaction between cyt. c and ITO electrode occurred. About 30% of a monolayer coverage was estimated from the coulomb amount in the surface area of oxidation and reduction peaks on cyclic voltammetry (CV) data. CV peak current maintained 84% for ITO electrode modified with 10-CDPA SAM film after 60 min continuous scan with 0.1 V/sec from 0.3 and -0.3 V vs. Ag/AgCl.},
keywords={},
doi={10.1587/transele.E98.C.152},
ISSN={1745-1353},
month={February},}
Copy
TY - JOUR
TI - extit{In situ} Observation of Direct Electron Transfer Reaction of Cytochrome extit{c} Immobilized on ITO Electrode Modified with 10-carboxydecylphosphonic Acid by Slab Optical Waveguide Spectroscopy and Cyclic Voltammetry
T2 - IEICE TRANSACTIONS on Electronics
SP - 152
EP - 155
AU - Naoki MATSUDA
AU - Hirotaka OKABE
PY - 2015
DO - 10.1587/transele.E98.C.152
JO - IEICE TRANSACTIONS on Electronics
SN - 1745-1353
VL - E98-C
IS - 2
JA - IEICE TRANSACTIONS on Electronics
Y1 - February 2015
AB - To immobilize cytochrome c (cyt. c) on ITO electrode with keeping its direct electron transfer (DET) activity, 10-carboxydecylphosphonic acid (10-CDPA) self-assembled monolayer (SAM) film was formed on ITO electrode. After 100 times washing process with exchanging phosphate buffer saline solution in the cell to fresh one, in situ slab optical waveguide (SOWG) absorption spectral measurement proved that about 80% of cyt. c immobilized on 10-CDPA modified ITO electrode was adsorbed on ITO electrode. Additionally SOWG spectral change of cyt. c between oxidized and reduced forms was observed with setting the ITO electrode potential at 0.3 and -0.3 V vs. Ag/AgCl, respectively showing DET reaction between cyt. c and ITO electrode occurred. About 30% of a monolayer coverage was estimated from the coulomb amount in the surface area of oxidation and reduction peaks on cyclic voltammetry (CV) data. CV peak current maintained 84% for ITO electrode modified with 10-CDPA SAM film after 60 min continuous scan with 0.1 V/sec from 0.3 and -0.3 V vs. Ag/AgCl.
ER -
English
