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Stem Cell Quantity Determination in Artificial Culture Bone by Ultrasonic Testing

Naomi YAGI, Tomomoto ISHIKAWA, Yutaka HATA

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Summary :

This paper describes an ultrasonic system that estimates the cell quantity of an artificial culture bone, which is effective for appropriate treat with a composite of this material and Bone Marrow Stromal Cells. For this system, we examine two approaches for analyzing the ultrasound waves transmitted through the cultured bone, including stem cells to estimate cell quantity: multiple regression and fuzzy inference. We employ two characteristics from the obtained wave for applying each method. These features are the amplitude and the frequency; the amplitude is measured from the obtained wave, and the frequency is calculated by the cross-spectrum method. The results confirmed that the fuzzy inference method yields the accurate estimates of cell quantity in artificial culture bone. Using this ultrasonic estimation system, the orthopaedic surgeons can choose the composites that contain favorable number of cells before the implantation.

Publication
IEICE TRANSACTIONS on Fundamentals Vol.E97-A No.4 pp.913-922
Publication Date
2014/04/01
Publicized
Online ISSN
1745-1337
DOI
10.1587/transfun.E97.A.913
Type of Manuscript
PAPER
Category
Ultrasonics

Authors

Naomi YAGI
  University of Hyogo
Tomomoto ISHIKAWA
  Ishikawa Hospital
Yutaka HATA
  University of Hyogo,Osaka University

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