Quantitative Analysis for Intracellular Distribution of a Photosensitizer Using Confocal Laser Scanning Microscope

Tomokazu NAGAO; Kazuki MATSUZAKI; Miho TAKAHASHI; Yoshiharu IMAZEKI; Haruyuki MINAMITANI

Quantitative Analysis for Intracellular Distribution of a Photosensitizer Using Confocal Laser Scanning Microscope

Tomokazu NAGAO, Kazuki MATSUZAKI, Miho TAKAHASHI, Yoshiharu IMAZEKI, Haruyuki MINAMITANI

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Confocal laser scanning microscope (CLSM) is capable of delivering a high axial resolution, and with this instrument even thin layers of cells can be imaged in good quality. Therefore, intracellular uptake and distribution properties of photosensitizer zinc coproporphyrin III tetrasodium salt (Zn CP-III) in human lung small cell carcinoma (Ms-1) were examined by using CLSM. In particular, the uptake of Zn CP-III in cytoplasm, plasma membrane, and nucleus was individually evaluated for the first time from fluorescence images obtained by CLSM. The results show that the Zn CP-III content in three cellular areas correlates with extracellular Zn CP-III concentration and time of incubation with Zn CP-III. Furthermore, it was found that the cytoplasmic fluorescence was approximately two times higher than that in the nucleus under all uptake conditions. In addition, cellular accumulation of Zn CP-III was compared with photodynamic cytotoxicity. The photocytotoxicity was to a great extent dependent on the uptake of the photosensitizer. The damaged site of Ms-1 cells induced by photodynamic therapy was plasma membrane. However, the content of Zn CP-III accumulated in cytoplasm was the highest among the three areas, implying that, besides the direct damage on plasma membrane, an oxidative damage to cellular component arose from the cytoplasmic Zn CP-III may also play an important role in photocytotoxicity. The quantitative information obtained in this study will be useful for further investigation of the photocytotoxicity as well as the uptake mechanism of photosensitizer.

Publication: IEICE TRANSACTIONS on Information Vol.E85-D No.1 pp.152-159

Publication Date: 2002/01/01

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Type of Manuscript: Special Section PAPER (Special Issue on Measurements and Visualization Technology of Biological Information)

Category: Cellular Imaging

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Tomokazu NAGAO, Kazuki MATSUZAKI, Miho TAKAHASHI, Yoshiharu IMAZEKI, Haruyuki MINAMITANI, "Quantitative Analysis for Intracellular Distribution of a Photosensitizer Using Confocal Laser Scanning Microscope" in IEICE TRANSACTIONS on Information, vol. E85-D, no. 1, pp. 152-159, January 2002, doi: .
Abstract: Confocal laser scanning microscope (CLSM) is capable of delivering a high axial resolution, and with this instrument even thin layers of cells can be imaged in good quality. Therefore, intracellular uptake and distribution properties of photosensitizer zinc coproporphyrin III tetrasodium salt (Zn CP-III) in human lung small cell carcinoma (Ms-1) were examined by using CLSM. In particular, the uptake of Zn CP-III in cytoplasm, plasma membrane, and nucleus was individually evaluated for the first time from fluorescence images obtained by CLSM. The results show that the Zn CP-III content in three cellular areas correlates with extracellular Zn CP-III concentration and time of incubation with Zn CP-III. Furthermore, it was found that the cytoplasmic fluorescence was approximately two times higher than that in the nucleus under all uptake conditions. In addition, cellular accumulation of Zn CP-III was compared with photodynamic cytotoxicity. The photocytotoxicity was to a great extent dependent on the uptake of the photosensitizer. The damaged site of Ms-1 cells induced by photodynamic therapy was plasma membrane. However, the content of Zn CP-III accumulated in cytoplasm was the highest among the three areas, implying that, besides the direct damage on plasma membrane, an oxidative damage to cellular component arose from the cytoplasmic Zn CP-III may also play an important role in photocytotoxicity. The quantitative information obtained in this study will be useful for further investigation of the photocytotoxicity as well as the uptake mechanism of photosensitizer.
URL: https://global.ieice.org/en_transactions/information/10.1587/e85-d_1_152/_p

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@ARTICLE{e85-d_1_152,
author={Tomokazu NAGAO, Kazuki MATSUZAKI, Miho TAKAHASHI, Yoshiharu IMAZEKI, Haruyuki MINAMITANI, },
journal={IEICE TRANSACTIONS on Information},
title={Quantitative Analysis for Intracellular Distribution of a Photosensitizer Using Confocal Laser Scanning Microscope},
year={2002},
volume={E85-D},
number={1},
pages={152-159},
abstract={Confocal laser scanning microscope (CLSM) is capable of delivering a high axial resolution, and with this instrument even thin layers of cells can be imaged in good quality. Therefore, intracellular uptake and distribution properties of photosensitizer zinc coproporphyrin III tetrasodium salt (Zn CP-III) in human lung small cell carcinoma (Ms-1) were examined by using CLSM. In particular, the uptake of Zn CP-III in cytoplasm, plasma membrane, and nucleus was individually evaluated for the first time from fluorescence images obtained by CLSM. The results show that the Zn CP-III content in three cellular areas correlates with extracellular Zn CP-III concentration and time of incubation with Zn CP-III. Furthermore, it was found that the cytoplasmic fluorescence was approximately two times higher than that in the nucleus under all uptake conditions. In addition, cellular accumulation of Zn CP-III was compared with photodynamic cytotoxicity. The photocytotoxicity was to a great extent dependent on the uptake of the photosensitizer. The damaged site of Ms-1 cells induced by photodynamic therapy was plasma membrane. However, the content of Zn CP-III accumulated in cytoplasm was the highest among the three areas, implying that, besides the direct damage on plasma membrane, an oxidative damage to cellular component arose from the cytoplasmic Zn CP-III may also play an important role in photocytotoxicity. The quantitative information obtained in this study will be useful for further investigation of the photocytotoxicity as well as the uptake mechanism of photosensitizer.},
keywords={},
doi={},
ISSN={},
month={January},}

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TY - JOUR
TI - Quantitative Analysis for Intracellular Distribution of a Photosensitizer Using Confocal Laser Scanning Microscope
T2 - IEICE TRANSACTIONS on Information
SP - 152
EP - 159
AU - Tomokazu NAGAO
AU - Kazuki MATSUZAKI
AU - Miho TAKAHASHI
AU - Yoshiharu IMAZEKI
AU - Haruyuki MINAMITANI
PY - 2002
DO -
JO - IEICE TRANSACTIONS on Information
SN -
VL - E85-D
IS - 1
JA - IEICE TRANSACTIONS on Information
Y1 - January 2002
AB - Confocal laser scanning microscope (CLSM) is capable of delivering a high axial resolution, and with this instrument even thin layers of cells can be imaged in good quality. Therefore, intracellular uptake and distribution properties of photosensitizer zinc coproporphyrin III tetrasodium salt (Zn CP-III) in human lung small cell carcinoma (Ms-1) were examined by using CLSM. In particular, the uptake of Zn CP-III in cytoplasm, plasma membrane, and nucleus was individually evaluated for the first time from fluorescence images obtained by CLSM. The results show that the Zn CP-III content in three cellular areas correlates with extracellular Zn CP-III concentration and time of incubation with Zn CP-III. Furthermore, it was found that the cytoplasmic fluorescence was approximately two times higher than that in the nucleus under all uptake conditions. In addition, cellular accumulation of Zn CP-III was compared with photodynamic cytotoxicity. The photocytotoxicity was to a great extent dependent on the uptake of the photosensitizer. The damaged site of Ms-1 cells induced by photodynamic therapy was plasma membrane. However, the content of Zn CP-III accumulated in cytoplasm was the highest among the three areas, implying that, besides the direct damage on plasma membrane, an oxidative damage to cellular component arose from the cytoplasmic Zn CP-III may also play an important role in photocytotoxicity. The quantitative information obtained in this study will be useful for further investigation of the photocytotoxicity as well as the uptake mechanism of photosensitizer.
ER -