Structural Changes of Millipore DOPH Artificial Membrane with Calcium Ions--Possibility of Calcium Ion Sensor--

Junji ARISAWA; Kenji MISAWA

Structural Changes of Millipore DOPH Artificial Membrane with Calcium Ions--Possibility of Calcium Ion Sensor--

Junji ARISAWA, Kenji MISAWA

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The surface structure of an artificial membrane composed of a Millipore filter and a synthetic lipid analogue, dioleylphosphate (DOPH), immersed in KCl solution changes when CaCl₂ concentration reaches a critical value. Using a photomultiplier mounted on the eyepiece of a stereoscopic microscope the structure change can be measured quantitatively as an intensity variation of reflected light of the membrane surface. Calcium ions decreased finally the intensity to more or less 50 % as compared with the original hydrophilic state whose solution was only KCl. However, the degree of structure change was dependent on following conditions: adsorbed DOPH densities, solution temperatures and pH values. On the other hand, using the light reflectance curves obtained from the structural observations, an unknown CaCl₂ concentration in a KCl solution can be detected. Since the reflectance curve shifts widely with various densities of DOPH adsorbed in the Millipore filter, it is possible to detect the CaCl₂ concentration of wide range. The detection range was from 0.1 to 50 mM CaCl₂.

Publication: IEICE TRANSACTIONS on transactions Vol.E70-E No.5 pp.504-509

Publication Date: 1987/05/25

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Type of Manuscript: PAPER

Category: Instrumentation

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Junji ARISAWA
Kenji MISAWA

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Junji ARISAWA, Kenji MISAWA, "Structural Changes of Millipore DOPH Artificial Membrane with Calcium Ions--Possibility of Calcium Ion Sensor--" in IEICE TRANSACTIONS on transactions, vol. E70-E, no. 5, pp. 504-509, May 1987, doi: .
Abstract: The surface structure of an artificial membrane composed of a Millipore filter and a synthetic lipid analogue, dioleylphosphate (DOPH), immersed in KCl solution changes when CaCl₂ concentration reaches a critical value. Using a photomultiplier mounted on the eyepiece of a stereoscopic microscope the structure change can be measured quantitatively as an intensity variation of reflected light of the membrane surface. Calcium ions decreased finally the intensity to more or less 50 % as compared with the original hydrophilic state whose solution was only KCl. However, the degree of structure change was dependent on following conditions: adsorbed DOPH densities, solution temperatures and pH values. On the other hand, using the light reflectance curves obtained from the structural observations, an unknown CaCl₂ concentration in a KCl solution can be detected. Since the reflectance curve shifts widely with various densities of DOPH adsorbed in the Millipore filter, it is possible to detect the CaCl₂ concentration of wide range. The detection range was from 0.1 to 50 mM CaCl₂.
URL: https://global.ieice.org/en_transactions/transactions/10.1587/e70-e_5_504/_p

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@ARTICLE{e70-e_5_504,
author={Junji ARISAWA, Kenji MISAWA, },
journal={IEICE TRANSACTIONS on transactions},
title={Structural Changes of Millipore DOPH Artificial Membrane with Calcium Ions--Possibility of Calcium Ion Sensor--},
year={1987},
volume={E70-E},
number={5},
pages={504-509},
abstract={The surface structure of an artificial membrane composed of a Millipore filter and a synthetic lipid analogue, dioleylphosphate (DOPH), immersed in KCl solution changes when CaCl₂ concentration reaches a critical value. Using a photomultiplier mounted on the eyepiece of a stereoscopic microscope the structure change can be measured quantitatively as an intensity variation of reflected light of the membrane surface. Calcium ions decreased finally the intensity to more or less 50 % as compared with the original hydrophilic state whose solution was only KCl. However, the degree of structure change was dependent on following conditions: adsorbed DOPH densities, solution temperatures and pH values. On the other hand, using the light reflectance curves obtained from the structural observations, an unknown CaCl₂ concentration in a KCl solution can be detected. Since the reflectance curve shifts widely with various densities of DOPH adsorbed in the Millipore filter, it is possible to detect the CaCl₂ concentration of wide range. The detection range was from 0.1 to 50 mM CaCl₂.},
keywords={},
doi={},
ISSN={},
month={May},}

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TY - JOUR
TI - Structural Changes of Millipore DOPH Artificial Membrane with Calcium Ions--Possibility of Calcium Ion Sensor--
T2 - IEICE TRANSACTIONS on transactions
SP - 504
EP - 509
AU - Junji ARISAWA
AU - Kenji MISAWA
PY - 1987
DO -
JO - IEICE TRANSACTIONS on transactions
SN -
VL - E70-E
IS - 5
JA - IEICE TRANSACTIONS on transactions
Y1 - May 1987
AB - The surface structure of an artificial membrane composed of a Millipore filter and a synthetic lipid analogue, dioleylphosphate (DOPH), immersed in KCl solution changes when CaCl₂ concentration reaches a critical value. Using a photomultiplier mounted on the eyepiece of a stereoscopic microscope the structure change can be measured quantitatively as an intensity variation of reflected light of the membrane surface. Calcium ions decreased finally the intensity to more or less 50 % as compared with the original hydrophilic state whose solution was only KCl. However, the degree of structure change was dependent on following conditions: adsorbed DOPH densities, solution temperatures and pH values. On the other hand, using the light reflectance curves obtained from the structural observations, an unknown CaCl₂ concentration in a KCl solution can be detected. Since the reflectance curve shifts widely with various densities of DOPH adsorbed in the Millipore filter, it is possible to detect the CaCl₂ concentration of wide range. The detection range was from 0.1 to 50 mM CaCl₂.
ER -