With increasing technology scaling and the use of lower voltages, more research interest is being shown in variability-tolerant analog front end design. In this paper, we describe an adaptive amplitude control transmitter that is operated using differential signaling to reduce the temperature variability effect. It enables low power, low voltage operation by synergy between adaptive amplitude control and Vth temperature variation control. It is suitable for high-speed interface applications, particularly cable interfaces. By installing an aggressor circuit to estimate transmitter jitter and changing its frequency and activation rate, we were able to analyze the effects of the interface block on the input buffer and thence on the entire system. We also report a detailed estimation of the receiver clock-data recovery (CDR) operation for transmitter jitter estimation. These investigations provide suggestions for widening the eye opening of the transmitter.