Testing the radio frequency compatibility between Cn-band Satellite Navigation and Microwave Landing System (MLS) has included establishing a specific interference model and reporting the effect of such interference. This paper considers two interference scenarios according to the interfered system. By calculating the Power Flux Density (PFD) values, the interference for Cn-band satellite navigation downlink signal from several visible space stations on MLS service is evaluated. Simulation analysis of the interference for MLS DPSK-data word signal and scanning signal on Cn-band satellite navigation signal is based on the Spectral Separation Coefficient (SSC) and equivalent Carrier-to-Noise Ratio methodologies. Ground tests at a particular military airfield equipped with MLS ground stations were successfully carried out, and some measured data verified the theoretical and numerical results. This study will certainly benefit the design of Cn-band satellite navigation signals and guide the interoperability and compatibility research of Cn-band satellite navigation and MLS.