In this paper, we propose improved methods of liquid-phase detection of biological targets utilizing magnetic markers and a high-critical-temperature superconducting quantum interference device (SQUID). For liquid-phase detection, the bound and unbound (free) markers are magnetically distinguished by using Brownian relaxation of free markers. Although a signal from the free markers is zero in an ideal case, it exists in a real sample on account of the aggregation and precipitation of free markers. This signal is called a blank signal, and it degrades the sensitivity of target detection. To solve this problem, we propose improved detection methods. First, we introduce a reaction field, Bre, during the binding reaction between the markers and targets. We additionally introduce a dispersion process after magnetization of the bound markers. Using these methods, we can obtain a strong signal from the bound markers without increasing the aggregation of the free markers. Next, we introduce a field-reversal method in the measurement procedure to differentiate the signal from the markers in suspension from that of the precipitated markers. Using this procedure, we can eliminate the signal from the precipitated markers. Then, we detect biotin molecules by using these methods. In an experiment, the biotins were immobilized on the surfaces of large polymer beads with diameters of 3.3 µm. They were detected with streptavidin-conjugated magnetic markers. The minimum detectable molecular number concentration was 1.8×10-19 mol/ml, which indicates the high sensitivity of the proposed method.

Electric fishes generate an AC electric field around themselves by the electric organ in the tail. Spatial distortion of the field by nearby objects is detected by an electroreceptor array located an over the body surface to localize the object electrically when other senses such as vision and mechanosense are useless. Each fish has its own 'frequency band' for its electric organ discharges, and jamming of the electrolocation system occurs when two fish with similar discharge frequencies encounter. To avoid janmming, the fish shift their discharge frequencies in appropriate directions. A computational algorithm for this electrical behavior and its neuronal implementation by the brain have been discovered. The design features of the system, however, are rather complex for this simple behavior and cannot be readily explained by functional optimization processes during evolution. To gain insights into the origin of the design features, two independently evolved electric fish species which perform the same behavior are compared. Complex features of the neuronal computation may be explained by the evolutionary history of neuronal elements.

For IF direct sampling phase detection method (IFSM) which realizes the arithmetical operations with digital filters by direct A/D (Analog to Digital) conversion of IF (Intermediate Frequency) signal, the method to eliminate DC offset is proposed and developed by using the gate array. A principle of the proposed method and the results of the measurement are shown.