Adsorption of Antibody Protein onto Plasma-Polymerized Film Characterized by Atomic Force Microscopy and Quartz Crystal Microbalance
Summary :
Adsorption of antibody protein (anti-human IgG) onto plasma-polymerized thin films (PPF) with nanoscale thickness was characterized by atomic force microscopy (AFM) and quartz crystal microbalance (QCM). The PPF surface is very flat (less than 1 nm roughness) and its properties (charge and wettability) can be easily changed while retaining the backbone structure. We focus on two types of surfaces: one is the pristine surface of hexamethyldisiloxane (HMDS) PPF (hydrophobic) and the other is an HMDS PPF surface with nitrogen-plasma treatment (hydrophilic and positive-charged surface). The AFM image showed that the antibody molecules were densely adsorbed onto both surfaces and individual antibody molecules could be observed. The QCM profiles show a corresponding tendency with the AFM images. These results indicate that the plasma polymerized film can be the suitable biointerface for the application of biosensor and bioassay.
- Publication
- IEICE TRANSACTIONS on Electronics Vol.E90-C No.3 pp.649-651
- Publication Date
- 2007/03/01
- Publicized
- Online ISSN
- 1745-1353
- DOI
- 10.1093/ietele/e90-c.3.649
- Type of Manuscript
- LETTER
- Category
- Organic Molecular Electronics
Authors
Keyword
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Hitoshi MUGURUMA, Satoshi MIURA, Naoya MURATA, "Adsorption of Antibody Protein onto Plasma-Polymerized Film Characterized by Atomic Force Microscopy and Quartz Crystal Microbalance" in IEICE TRANSACTIONS on Electronics,
vol. E90-C, no. 3, pp. 649-651, March 2007, doi: 10.1093/ietele/e90-c.3.649.
Abstract: Adsorption of antibody protein (anti-human IgG) onto plasma-polymerized thin films (PPF) with nanoscale thickness was characterized by atomic force microscopy (AFM) and quartz crystal microbalance (QCM). The PPF surface is very flat (less than 1 nm roughness) and its properties (charge and wettability) can be easily changed while retaining the backbone structure. We focus on two types of surfaces: one is the pristine surface of hexamethyldisiloxane (HMDS) PPF (hydrophobic) and the other is an HMDS PPF surface with nitrogen-plasma treatment (hydrophilic and positive-charged surface). The AFM image showed that the antibody molecules were densely adsorbed onto both surfaces and individual antibody molecules could be observed. The QCM profiles show a corresponding tendency with the AFM images. These results indicate that the plasma polymerized film can be the suitable biointerface for the application of biosensor and bioassay.
URL: https://global.ieice.org/en_transactions/electronics/10.1093/ietele/e90-c.3.649/_p
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@ARTICLE{e90-c_3_649,
author={Hitoshi MUGURUMA, Satoshi MIURA, Naoya MURATA, },
journal={IEICE TRANSACTIONS on Electronics},
title={Adsorption of Antibody Protein onto Plasma-Polymerized Film Characterized by Atomic Force Microscopy and Quartz Crystal Microbalance},
year={2007},
volume={E90-C},
number={3},
pages={649-651},
abstract={Adsorption of antibody protein (anti-human IgG) onto plasma-polymerized thin films (PPF) with nanoscale thickness was characterized by atomic force microscopy (AFM) and quartz crystal microbalance (QCM). The PPF surface is very flat (less than 1 nm roughness) and its properties (charge and wettability) can be easily changed while retaining the backbone structure. We focus on two types of surfaces: one is the pristine surface of hexamethyldisiloxane (HMDS) PPF (hydrophobic) and the other is an HMDS PPF surface with nitrogen-plasma treatment (hydrophilic and positive-charged surface). The AFM image showed that the antibody molecules were densely adsorbed onto both surfaces and individual antibody molecules could be observed. The QCM profiles show a corresponding tendency with the AFM images. These results indicate that the plasma polymerized film can be the suitable biointerface for the application of biosensor and bioassay.},
keywords={},
doi={10.1093/ietele/e90-c.3.649},
ISSN={1745-1353},
month={March},}
Copy
TY - JOUR
TI - Adsorption of Antibody Protein onto Plasma-Polymerized Film Characterized by Atomic Force Microscopy and Quartz Crystal Microbalance
T2 - IEICE TRANSACTIONS on Electronics
SP - 649
EP - 651
AU - Hitoshi MUGURUMA
AU - Satoshi MIURA
AU - Naoya MURATA
PY - 2007
DO - 10.1093/ietele/e90-c.3.649
JO - IEICE TRANSACTIONS on Electronics
SN - 1745-1353
VL - E90-C
IS - 3
JA - IEICE TRANSACTIONS on Electronics
Y1 - March 2007
AB - Adsorption of antibody protein (anti-human IgG) onto plasma-polymerized thin films (PPF) with nanoscale thickness was characterized by atomic force microscopy (AFM) and quartz crystal microbalance (QCM). The PPF surface is very flat (less than 1 nm roughness) and its properties (charge and wettability) can be easily changed while retaining the backbone structure. We focus on two types of surfaces: one is the pristine surface of hexamethyldisiloxane (HMDS) PPF (hydrophobic) and the other is an HMDS PPF surface with nitrogen-plasma treatment (hydrophilic and positive-charged surface). The AFM image showed that the antibody molecules were densely adsorbed onto both surfaces and individual antibody molecules could be observed. The QCM profiles show a corresponding tendency with the AFM images. These results indicate that the plasma polymerized film can be the suitable biointerface for the application of biosensor and bioassay.
ER -
English
